HiFi Sequencing of Cannabis sativa L and other medicinal fungi Accelerated breeding [bbLpUjhuoZt]
Presented By: Kevin McKernan
Speaker Biography: Kevin is the CSO and Founder of Medicinal Genomics and has pioneered the genomics of cannabis and hemp to build a stronger scientific environment (Kannapedia.net) for the study of cannabis based therapeutics and blockchain technologies for tracking and verifying cannabis genetics. Previously, Kevin was the CSO of Courtagen Life Sciences, Inc., and was Vice President and Director of R&D of Life Technologies where he managed the development of Life Technologies next generation SOLiD sequencing technology. Integral to the SOLiD R&D process, Kevin oversaw over 100 research collaborations exploring the new biological frontiers with next generation sequencing and saw particular excitement and traction in human tumor sequencing. Kevin initiated an R&D project to investigate chemFET semiconductor based DNA sequencing and spearheaded a process to acquire the DNA sequencing company Ion Torrent for $350M. These collaborations resulted in hundreds of publications and 7 Journal covers from Science Translational Medicine to Nature.
Webinar: HiFi Sequencing of Cannabis sativa L. and other medicinal fungi: Accelerated breeding
Webinar Abstract: The utilization of high fidelity long read sequencing is critical for the resolution of repetitive genomes. Cannabis sativa is known to be diploid (2n=20), 70% repetitive, 64% AT rich and 1% polymorphic. The chemotypic genes (CBDAS, THCAS, CBCAS) governing cannabinoid synthesis are buried in multiple megabase LTR forests. High fidelity long read sequencers resolve these repetitive regions and enable genotypic classification of chemical expression. Pathogen resistance genes are equally important to the prediction of cannabinoid yield. Utilizing Pacific Biosciences HiFi sequencing, we construct reference grade genomes that enable population surveys of diverse chemotypes and pathogen resistant cultivars. We demonstrate the use of HiFi reads to resolve both complex medicinal plant genomes like Cannabis sativa L. and the rapid resolution of the medicinal fungal genome Psilocybe cubensis.
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